Table 1.

Absence of Apoptosis Inhibition of PEN and LPS-treated PBN in Both A1-a^{− /−} and A1-a^{+ /−} Mice

Genotype		Source of neutrophils		Treatment		% Apoptotic cells ± SE				P value using the unpaired t test (12-h incubation)
						Incubation time
						6 h		12 h		vs. A1-a+/−		vs. Wild-type
A1-a−/−		PBN		NT*		7.1 ± 0.8		20.6 ± 1.9		<0.001		<0.001
A1-a−/−		PBN		LPS‡		4.9 ± 0.7		19.0 ± 1.1		<0.001		<0.001
A1-a−/−		PEN		NT		7.8 ± 1.2		18.6 ± 1.3		<0.001		<0.001
A1-a+/−		PBN		NT		3.3 ± 0.5		8.3 ± 1.2				0.22
A1-a+/−		PBN		LPS		3.7 ± 0.5		9.2 ± 1.0				<0.05
A1-a+/−		PEN		NT		5.1 ± 1.8		11.0 ± 2.2				<0.05
Wild-type		PBN		NT		5.0 ± 0.4		10.4 ± 0.8
Wild-type		PBN		LPS		2.4 ± 1.0§		5.2 ± 1.4§
Wild-type		PEN		NT		1.4 ± 0.2§		3.8 ± 0.4§

Neutrophils from the indicated source were incubated for 6 or 12 h. Experiments were conducted five times.  

^*  NT, not treated.  

^‡ Neutrophils were incubated in the presence of LPS (10 μg/ml).  

^§  P < 0.01 compared with PBN NT* by the unpaired t test.