Figure 6.

Stability of TCR sequences after viral clearance. DNA sequences and deduced amino acid sequences of the Vβ8.1-Jβ1.6 and Vβ8.1-Jβ1.5 were derived from direct sequencing of PCR DNA. The sequences shown represent the deduced amino acid sequences of the VDJ junctions beginning with residue 92 (33) of the amplified PCR products that were rearranged to the Jβ1.5 and Jβ1.6 gene segments. PCR products were amplified from the same individual mice at various time points after LCMV infection and then subjected to spectratyping. Discrete spectratype peaks were selected for direct DNA sequencing as described in Materials and Methods. The d8 and d12 DNA sequences from mice 1–4 were derived by using the same PCR products used for spectratyping in Fig. 5. At some positions it was clear from the DNA sequence data that either of two amino acids were encoded by what were probably two clones, and these are designated in the A/B format. The unreadable DNA sequences and amino acid translation in mouse 2 were designated N and X, respectively.