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. 1998 Dec 7;188(11):1993–2005. doi: 10.1084/jem.188.11.1993

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Skewed Vβ8.1 spectratype within CD8⁺ T cells under conditions of clonal exhaustion by high dose LCMV clone 13 infection. The representative CDR3 profiles were obtained from PB and sorted spleen cells at 13 d after LCMV clone 13 infection. The spleen cells were sorted by FACS^® to obtain CD4⁺ and CD8⁺ T cells. The purities of the sorted CD4⁺ and CD8⁺ T cells were 88 and 95%, respectively. Total RNA was extracted from each population of T cells and subjected to spectratyping.