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. 1998 Dec 7;188(11):1977–1983. doi: 10.1084/jem.188.11.1977

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OVA_257–264-coated B cells induce OVA-specific CTL tolerance in B6 mice. B6 mice were injected intravenously with 10⁷ OVA_257–264-coated B6 B cells, unpulsed B6 B cells, or medium alone, and rechallenged 7 d later with (a and c) 25 × 10⁶ OVA-loaded spleen cells injected intravenously or (b) 10 μg OVA_257–264in CFA injected subcutaneously. After an additional 7 d, their spleens were removed and stimulated in vitro for 6 d before a 4-h chromium release assay was performed. (a and b) Percent specific lysis of ⁵¹Cr-labeled OVA_257–264-coated EL4 (filled symbols) and EL4 (open symbols) in mice injected with B cells coated with OVA_257–264 at 10 μg/ml (squares) or with medium alone (circles). (c) The number of OVA-specific LU/spleen for individual mice (filled triangles) treated as in a, and calculated as described in Materials and Methods. OVAp, OVA_257–264 coated. Open circles, average LU/spleen for each group.

OVA_257–264-coated B cells induce OVA-specific CTL tolerance in B6 mice. B6 mice were injected intravenously with 10⁷ OVA_257–264-coated B6 B cells, unpulsed B6 B cells, or medium alone, and rechallenged 7 d later with (a and c) 25 × 10⁶ OVA-loaded spleen cells injected intravenously or (b) 10 μg OVA_257–264in CFA injected subcutaneously. After an additional 7 d, their spleens were removed and stimulated in vitro for 6 d before a 4-h chromium release assay was performed. (a and b) Percent specific lysis of ⁵¹Cr-labeled OVA_257–264-coated EL4 (filled symbols) and EL4 (open symbols) in mice injected with B cells coated with OVA_257–264 at 10 μg/ml (squares) or with medium alone (circles). (c) The number of OVA-specific LU/spleen for individual mice (filled triangles) treated as in a, and calculated as described in Materials and Methods. OVAp, OVA_257–264 coated. Open circles, average LU/spleen for each group.