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. 1998 Dec 7;188(11):1977–1983. doi: 10.1084/jem.188.11.1977

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OT-I cells remaining at day 14 in B cell–treated mice possess full CTL effector function. 14 d after immunization with OVA_257–264-coated B cells (squares) or medium alone (circles), the LN cells from adoptively transferred (B6 × B6. Kathy)F₁ host mice were titrated into 200 μl microcultures and stimulated for 5 d with irradiated OVA-loaded spleen cells. The number of responder OT-I cells per well was determined by flow cytometry. The cytotoxicity was then measured in a 4-h chromium release assay using ⁵¹Cr-labeled OVA_257–264-coated EL4 (filled symbols) and uncoated EL4 (open symbols) targets. This experiment was performed four times with two to four mice per group.