Figure 8.

Restoration of EAE in Ig–PLP1 tolerized mice by administration of anti–IL-4 antibody. Newborn mice were injected intraperitoneally within 24 h of birth with 100 μg Ig–PLP1 in saline. When they reached 7 wk of age, a group of seven mice was injected intraperitoneally with 1 mg/mouse of affinity purified 11B11 anti–IL-4 antibody in 500 μl of PBS (□). A second group of five mice was injected with 1 mg/mouse of rat IgG in 500 μl PBS (⋄) to serve as control. On the next day all mice were induced for EAE with PLP1 peptide as described in Fig. 1. 5 d after disease induction, the mice were given a second injection of 1 mg/mouse intraperitoneally of 11B11 or rat IgG. The mice were scored daily for signs of paralysis. For comparison purposes the clinical scores of the mice described in Fig. 1 that were tolerized with Ig–PLP1 (▴) or Ig–W (○) at birth and induced for EAE with PLP1 peptide at 7 wk of age were included.