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. 1998 Nov 16;188(10):1907–1916. doi: 10.1084/jem.188.10.1907

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Secreted protein profiles of REPEC O103, RDEC-1, EPEC (A), and REPEC O103 mutant strains (B). Bacteria were grown in DMEM and the secreted proteins were precipitated by addition of 10% TCA, and then analyzed by 12% SDS-PAGE stained with Coomassie blue. Strains: REPEC O103, O103; AAF004 (EspA⁻), ΔespA; AAF005 (EspB⁻), ΔespB.