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. 1998 Nov 16;188(10):1907–1916. doi: 10.1084/jem.188.10.1907

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Accumulation of cytoskeletal actin and tyrosine-phosphorylated proteins beneath bacteria in cultured epithelial cells. HeLa cells (10⁵) were infected for 3 h with REPEC O103 or isogenic mutant strains. Infected HeLa cells were fixed by the addition of paraformaldehyde, then phalloidin-TxR and antiphosphotyrosine antibodies (αPY) were used for detection of colocalization of actin and tyrosine-phosphorylated proteins by immunofluorescence microscopy. Strains: REPEC O103, O103; AAF004 (EspA⁻), ΔespA; AAF005 (EspB⁻), ΔespB.