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. 1998 Nov 16;188(10):1907–1916. doi: 10.1084/jem.188.10.1907

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Confocal laser scanning micrographs of Peyer's patches infected with REPEC O103, AAF004, and AAF005. Peyer's patches were taken 72 h after infection and cryosections (20-μm-thick) were stained with phalloidin-TxR (red for overlay) and anti-O103 antibody (green for overlay). REPEC O103 adhered and colonized to the Peyer's patches, cytoskeletal actin beneath the attached bacteria was rearranged, and cup-like structures were also observed. In contrast, a small number of EspA and EspB mutant strains were observed in villi crypts, but no actin rearrangements occurred. Strains: REPEC O103, O103; AAF004 (EspA⁻), ΔespA; AAF005 (EspB⁻), ΔespB; PBS inoculation, Control.