Figure 3.

The mononuclear cell infiltrate seen in the glomeruli and renal cortex in normal HN at 12 wk was prevented by early CD8⁺ T cell depletion and significantly attenuated by late CD8⁺ T cell depletion. (a) A glomerular infiltrate of α/β-TCR⁺ (R73), CD4⁺ (W3/25), and CD8⁺ (MRC OX-8) cells, and macrophages (ED-1), but not NK cells (3.2.3), was present in normal HN at 12 wk. This infiltrate was not present in early or late CD8-depleted HN. Values represent the mean ± SEM (n = 5) of cells per glomerulus (10 glomeruli per rat). (b) A renal cortical infiltrate of α/β-TCR⁺, CD4⁺, and CD8⁺ cells, and macrophages, but not NK cells, was present in untreated HN at 12 wk. This infiltrate was absent in early CD8-depleted HN. In late CD8-depleted HN there was a small infiltrate staining only for R73 and ED-1. Values represent the mean ± SEM (n = 5) of cells per HPF (10 HPF per rat). *P < 0.01, **P < 0.001, ***P < 0.0001. ANOVA followed by Bonferroni-Dunn post hoc multiple comparisons test.