Figure 5.

At 12 wk, CD8⁺ T cell depletion prevented the increase in Th1, cytotoxic T cell, and macrophage cytokine expression in glomeruli from rats with HN. Significant increases in cytokine mRNA expression for Th1 cells, cytotoxic cells, and macrophages were seen in normal HN compared with the background expression in CFA controls. These increases were not detected in early and late CD8-depleted glomeruli. Cytotoxic cell molecules in rats depleted of CD8⁺ T cells were probably from NK cells. Results expressed as the median cycle number (n = 4) at which PCR product was first detected, measured at five-cycle intervals. *P < 0.01, **P < 0.001, ***P < 0.0001. U, PCR product undetectable. ANOVA followed by Bonferroni-Dunn post hoc multiple comparisons test.