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. 1998 Dec 21;188(12):2369–2374. doi: 10.1084/jem.188.12.2369

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(A) Analysis of homologous recombinant ES cell clones by Southern blot using an sγ1 probe. EcoRI digestion of genomic DNA yielded fragment sizes of 13.0 and 8.0 kb of the wild-type (+) and Ad-hMT (Ad) alleles, respectively. (B) Analysis of heterozygous and homozygous targeted mice and C57BL/6 wild-type littermates. Agarose gel electrophoresis of PCR products of tail DNA amplified with primers 1, 2, and 3.

(A) Analysis of homologous recombinant ES cell clones by Southern blot using an sγ1 probe. EcoRI digestion of genomic DNA yielded fragment sizes of 13.0 and 8.0 kb of the wild-type (+) and Ad-hMT (Ad) alleles, respectively. (B) Analysis of heterozygous and homozygous targeted mice and C57BL/6 wild-type littermates. Agarose gel electrophoresis of PCR products of tail DNA amplified with primers 1, 2, and 3.