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. 1998 Dec 21;188(12):2313–2320. doi: 10.1084/jem.188.12.2313

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Autologous apoptotic cells activate serum complement and are coated with the C3 breakdown product, C3bi. Lymphocytes (A–D) or neutrophils (E) were untreated (A) or induced to undergo apoptosis (B–E) and incubated with 15% autologous serum for 1 h as described in Materials and Methods. Lymphocytes were stained with PE-conjugated murine anti–human-C3bi (Quidel; bold line) or PE-IgG_2bκ as isotype control (dotted line). In C and D, apoptotic lymphocytes were incubated with 0.4 or 1.6 mM annexin V, respectively (provided by Dr. A.E. Gharavi, Louisiana State University Medical Center, New Orleans, LA), for 10 min at 4°C followed by incubation with RPMI containing 15% autologous serum for 1 h/37°C/5% CO₂, and then stained for C3bi as in A and B. In E, neutrophils were induced to undergo apoptosis, incubated with 15% autologous serum for 1 h, and then analyzed by two-color flow cytometry as shown. The percent staining in each compartment is shown. These results are representative of four experiments.

Autologous apoptotic cells activate serum complement and are coated with the C3 breakdown product, C3bi. Lymphocytes (A–D) or neutrophils (E) were untreated (A) or induced to undergo apoptosis (B–E) and incubated with 15% autologous serum for 1 h as described in Materials and Methods. Lymphocytes were stained with PE-conjugated murine anti–human-C3bi (Quidel; bold line) or PE-IgG_2bκ as isotype control (dotted line). In C and D, apoptotic lymphocytes were incubated with 0.4 or 1.6 mM annexin V, respectively (provided by Dr. A.E. Gharavi, Louisiana State University Medical Center, New Orleans, LA), for 10 min at 4°C followed by incubation with RPMI containing 15% autologous serum for 1 h/37°C/5% CO₂, and then stained for C3bi as in A and B. In E, neutrophils were induced to undergo apoptosis, incubated with 15% autologous serum for 1 h, and then analyzed by two-color flow cytometry as shown. The percent staining in each compartment is shown. These results are representative of four experiments.