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. 1998 Dec 21;188(12):2313–2320. doi: 10.1084/jem.188.12.2313

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The complement receptors CR3 and CR4 are predominantly responsible for uptake of apoptotic cells in the presence of serum. (A) The expression of the complement receptors was examined on day 7 human macrophages by flow cytometry using specific mouse mAbs (black histogram) as described in Materials and Methods. The percentage of positive cells compared with the appropriate isotype control (grey histogram) is shown for each antibody. (B) Macrophages were preincubated with the antibody or tetrapeptide shown for 15 min at 4°C in the presence of 15% autologous human serum. The PI in the presence of the inhibitor was calculated from three separate experiments, and results were compared with the serum control by Student's t test. Inhibition by antibodies to CR3 (P = 0.0001), CR4 (P = 0.0001), annexin V (ANN; P = 0.001), and RGDS (P = 0.01) was statistically significant, whereas neither anti-CR1, anti-CD36, anti-CD14 (61D3 and 63D3), nor the control tetrapeptide (RGES) significantly inhibited uptake in the presence of serum.

The complement receptors CR3 and CR4 are predominantly responsible for uptake of apoptotic cells in the presence of serum. (A) The expression of the complement receptors was examined on day 7 human macrophages by flow cytometry using specific mouse mAbs (black histogram) as described in Materials and Methods. The percentage of positive cells compared with the appropriate isotype control (grey histogram) is shown for each antibody. (B) Macrophages were preincubated with the antibody or tetrapeptide shown for 15 min at 4°C in the presence of 15% autologous human serum. The PI in the presence of the inhibitor was calculated from three separate experiments, and results were compared with the serum control by Student's t test. Inhibition by antibodies to CR3 (P = 0.0001), CR4 (P = 0.0001), annexin V (ANN; P = 0.001), and RGDS (P = 0.01) was statistically significant, whereas neither anti-CR1, anti-CD36, anti-CD14 (61D3 and 63D3), nor the control tetrapeptide (RGES) significantly inhibited uptake in the presence of serum.