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. 1998 Dec 21;188(12):2321–2333. doi: 10.1084/jem.188.12.2321

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Production of CD4^+/L mice. A 326-bp fragment containing most of exons 2 and 3 (including a 30-bp intron) was deleted and replaced by the βgeo and neo cassettes. The 5′ external probe, an XhoI-AvrII fragment, detects an endogenous 15-kb XhoI band on Southern blots of DNA from wild-type D3 ES cells and a smaller 9.6-kb band on blots of DNA from cells carrying the knock-in mutation. Exons are marked with roman numerals, untranslated regions by open boxes, and translated regions by filled boxes. Arrows below the cassettes represent their 5′ to 3′ orientation. Bent arrows mark the transcription initiation sites. Restriction enzyme sites are abbreviated as follows: X (XhoI), B (BamHI), A (AvrII), S (SphI), H (HindIII), E (EcoRI), Sp (SpeI), Sm (SmaI), P (PstI), C (ClaI), Xb (XbaI), Sa (SalI), Sc (SacI), and Bg (BglII).