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. 1998 Dec 21;188(12):2321–2333. doi: 10.1084/jem.188.12.2321

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βgal⁺ DN cells in CD4-deficient mice. Thymocytes and lymph node cells from homozygous (CD4^0/L) and heterozygous (CD4^+/L) mice were compared using four-color flow cytometry. Cells were first plotted for their expression of βgal and CD8. CD8⁺βgal⁺ cells were gated and further evaluated for their expression of CD4 and CD3. Percentages are of total thymocytes.