Figure 2.

Long-term subcutaneous treatment with SCF increases the numbers of mast cells in the peritoneal lavage fluid, and survival after CLP, in wild-type Kit ^+/+ mice and in mast cell–reconstituted Kit^W/Kit^W-v mice, but not in mast cell–deficient Kit^W/Kit^W-v mice. (A) Some mice (male, 11–13 wk old at time of death or CLP) were killed after the last of 21 daily subcutaneous injections with vehicle or rrSCF-peg (30 μg/kg per day) for assessment of the numbers of mast cells in peritoneal lavage fluid. Numbers of mice per group are indicated inside the bars. (B) Some mice were treated daily for 21 d before, and for 14 d or until death after, CLP (∼50% ligation, single puncture with a 0.7-mm needle). P values in parentheses are for survival after day 3. Data were pooled from two independent experiments per treatment group. MC, mast cell; PLF, peritoneal lavage fluid.