Table 1.
Analyses of Peritoneal Lavage 3 h after CLP
| +/+ | −/− | −/− IgM | −/− PtC-IgM | −/− PC-IgM | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TNF-α (pg/ml) | 1,507 | 892 | 2,068 | 1,643 | 304 | |||||
| IL-6 (pg/ml) | 2,734 | 1,401 | ND | ND | ND | |||||
| Neutrophils (%) | 81.7 | 54.8 | 83.5 | 69.6 | 22.1 | |||||
| E. coli (CFU) | 34.9 × 103 | 350 × 103 | 2.3 × 103 | 62.5 × 103 | 510 × 103 | |||||
| LPS (EU/ml) | 36.7 | 62.9 | 23.8 | 51.2 | 64.0 |
sIgM-deficient mice (−/−), wild-type mice (+/+), and IgM-reconstituted sIgM-deficient mice were subject to CLP. Peritoneal lavage was carried out by injection of 3 ml i.p. of PBS with 2% FCS 3 h after CLP. IgM-reconstituted mice were given a single dose of 0.5 mg i.v. of purified polyclonal IgM from normal mouse serum or monoclonal IgM specific to PtC or PC 4 h before CLP. Peritoneal lavages of sIgM-deficient and wild-type mice were each pooled from seven mice, and the levels of TNF-α, IL-6, and LPS, E. coli counts, and neutrophils were assayed (see Materials and Methods). The levels of TNF-α and LPS, E. coli counts, and neutrophils were assayed in peritoneal lavage from individual IgM-reconstituted mice. The average of four mice is shown. Similar results were obtained in a separate experiment.