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. 1998 Jul 20;188(2):341–350. doi: 10.1084/jem.188.2.341

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Intrahepatic T cell and cytokine profiles in HBV transgenic mice. Total RNA was extracted from nontumorous liver tissue of representative mice from each group 17 mo after adoptive transfer (groups 1 and 2) and from unmanipulated age-matched controls (group 3), and subjected to RNase protection analysis to monitor the expression of CD3γ, CD4, CD8α, F480, and a panel of inflammatory cytokines. Lanes 19–21 contain total liver RNA from three HBV transgenic mice that were injected with an HBsAg-specific CTL clone which induced a severe transient acute hepatitis in transgenic recipients (references 26 and 28). The ribosomal protein light 32 (L32) RNA was used to normalize the amount of RNA loaded in each lane.