. 1998 Jul 20;188(2):277–286. doi: 10.1084/jem.188.2.277

Table 1.

IFN-γ Release by Peptide-stimulated Splenocytes from gp100-immunized Mice

Immunization	rVVhgp100			rVVmgp100			rVVEShgp100_25–33			rVVESmgp100_25–33			Naive
In vitrostimulation	rVVhgp100			rVVmgp100			rVVEShgp100_25–33			rVVESmgp100_25–33			Naive
In vitrostimulation	hgp	mgp	OVA	hgp	mgp	OVA	hgp	mgp	OVA	hgp	mgp	OVA	hgp	mgp
Peptide targets
None	264	584	236	137	0	51	279	428	5,297	862	1,082	1,324	215	456
hgp	15,120	20,751	250	137	1	0	15,945	11,197	1,267	1,295	1,580	819	229	428
mgp	2,567	19,307	257	94	58	37	940	11,268	1,438	911	847	1,629	215	527
OVA	165	343	144	137	37	208	172	449	300	648	698	2,141	257	478

Mice were vaccinated with indicated rVVs, and splenocytes were isolated 3 wk later and restimulated for 6 d with 1 μg/ml peptide. Subsequent stimulation of cultured cells with hgp100_25–33 or mgp100_25–33 peptide resulted in IFN-γ release only by splenocytes from mice immunized with rVVhgp100 or rVVEShgp100_25–33. hgp, hgp100 AA 25–33 (KVPRNQDWL); mgp, mgp100 AA 25–33 (EGSRNQDWL); OVA, ovalbumin, AA 257–264 (SIINFEKL). Four repeat experiments yielded similar results; numbers indicate IFN-γ (pg/ml) secreted by 2 × 10⁵ CTLs in 24 h; numbers in bold indicate secretion >3-fold over control peptide.