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. 1998 Jul 20;188(2):373–386. doi: 10.1084/jem.188.2.373

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CD11c⁺ peripheral blood DCs respond to MIP-3β but not to MIP-3α upon spontaneous maturation. CD11c⁺ DCs were FACS^® sorted from PBLs according to their lack of lineage markers and expression of CD4 and CD11c (see Materials and Methods). (A) The responses to MIP-3α (500 ng/ml) and to MIP-3β (500 ng/ml) were determined after FACS^® sorting and after 16-h culture with GM-CSF. Migration assays were performed in Boyden microchambers. Results are expressed as number of migrating cells per two low power fields (original magnification: ×20). Results are representative of four experiments. (B) The expression of CCR7 mRNA was assessed by semiquantitative RT-PCR. Results at 35 cycles are shown, and PCR for β actin is shown at 21 cycles as an internal mRNA control. Results are representative of three experiments.