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. 1998 Oct 5;188(7):1307–1320. doi: 10.1084/jem.188.7.1307

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Characterization of inflammatory cells in lung sections from an IL-9–expressing mouse. Formalin-fixed tissue was stained with new vital red to demonstrate that eosinophils were present in high numbers in lung tissue from IL-9–expressing mice (A). Immunostaining of frozen lung sections (B–E) revealed that cell infiltrates also contained CD4⁺ (B), CD8⁺ (C), B220⁺ (D), and Mac-1⁺ (E) mononuclear cells. Original magnifications: A, ×600; B–E, ×500.