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. 1998 Oct 5;188(7):1359–1368. doi: 10.1084/jem.188.7.1359

Figure 4.

Figure 4

Immature DCs but not mature DCs or macrophages cross-present antigenic material derived from apoptotic cells. Various populations of HLA-A2.1+ APCs were cocultured with HLA-A2.1− influenza-infected monocytes. After 12 h, the APCs were loaded with 51Cr and used as targets for HLA-A2.1+ influenza-reactive CTL lines. Mature DCs were isolated by labeling with the DC-restricted marker CD83, followed by cell sorting on the FACSort®. Immature DCs were CD14− and were sorted by FACSort® as a CD83− population. Mature macrophages were generated by culturing an adherent mononuclear cell fraction in a Teflon beaker for 9 d. Effector/target ratios = 45:1 and 15:1 (A). Controls included infected and uninfected mature DCs, immature DCs, and macrophages. The HLA-A2.1− monocytes used as a source of apoptotic material were also tested as targets to demonstrate the absence of lysis when using a mismatched target. Effector/target ratios = 45:1 and 15:1. Results are representative of three experiments and the values shown represent the mean of triplicate wells (B).