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. 1998 Oct 5;188(7):1359–1368. doi: 10.1084/jem.188.7.1359

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Intracellular but not extracellular CD83 expression distinguishes immature DCs from mature DCs and macrophages. Macrophages, immature DCs, and mature DCs were prepared as previously described. Cells were either untreated (Surface) or permeabilized with saponin (Intracellular), and incubated with anti-CD83. Cells were then labeled using a PE-conjugated GAM-Ig (Sigma Biosciences). An isotype-matched antibody was used (IgG2a) as a control.