Table 1.
Receptor Profile of Various APCs
| Immature DCs Day 7 | Mature DCs Day 11 | Monocytes Day 0 | Macrophages Day 3 | Macrophages Day 9 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| n = 7 | n = 6 | n = 4 | n = 5 | n = 5 | ||||||
| CD8* | 0–2 (3 ± 1)§ | 0–3 (4 ± 1) | 0–1 (4 ± 1) | 0–5 (3 ± 1) | 1–2 (3 ± 1) | |||||
| CD14* | 1–10 (4 ± 1) | 0–6 (4 ± 1) | 83–97 (149 ± 90) | 67–98 (61 ± 20) | 49–90 (68 ± 48) | |||||
| CD83‡ | 8–43 (8 ± 4) | 73–99 (114 ± 15) | 0–1 (3 ± 1) | 4–19 (4 ± 2) | 2–14 (4 ± 2) | |||||
| MHC I‡ | 99–100 (311 ± 49) | 96–100 (280 ± 69) | 99–100 (99 ± 38) | 97–99 (164 ± 24) | 95–100 (116 ± 18) | |||||
| HLA-DR* | 88–100 (84 ± 35) | 98–99 (188 ± 40) | 94–99 (63 ± 9) | 97–99 (173 ± 34) | 5–89 (25 ± 27) | |||||
| CD8‡ | 3–9 (4 ± 1) | 2–8 (3 ± 1) | 0–2 (3 ± 1) | 1–4 (3 ± 0) | 1–5 (3 ± 1) | |||||
| IgG2b‡ | 3–7 (3 ± 1) | 2–7 (3 ± 1) | 0–2 (3 ± 0) | 1–4 (3 ± 1) | 1–5 (3 ± 1) | |||||
| CD36‡ | 75–99 (75 ± 31) | 20–50 (9 ± 2) | 89–99 (62 ± 18) | 64–98 (69 ± 45) | 9–55 (15 ± 16) | |||||
| αv (CD51)‡ | 45–76 (14 ± 3) | 11–40 (9 ± 3) | 2–12 (4 ± 1) | 2–35 (6 ± 2) | 18–75 (10 ± 5) | |||||
| β1 (CD29)‡ | 99–100 (287 ± 57) | 84–100 (112 ± 28) | 95–100 (61 ± 33) | 83–100 (72 ± 23) | 68–99 (104 ± 29) | |||||
| β3 (CD61)‡ | 14–34 (7 ± 2) | 10–24 (6 ± 1) | 76–91 (40 ± 16)‖ | 15–85 (14 ± 9)‖ | 19–92 (16 ± 9) | |||||
| β5 ‡ | 78–95 (30 ± 8) | 32–57 (11 ± 2) | 6–8 (5 ± 1) | 8–15 (7 ± 2) | 5–8 (3 ± 1) | |||||
| αvβ3 (VnR)‡ | 22–28 (7 ± 1) | 14–30 (7 ± 2) | 4–8 (4 ± 1) | 7–27 (5 ± 2) | 19–68 (10 ± 4) | |||||
| αvβ5 ‡ | 81–90 (25 ± 5) | 12–53 (9 ± 3) | 1–14 (4 ± 1) | 0–11 (3 ± 1) | 6–15 (4 ± 1) | |||||
| CD71‡ | 75–99 (50 ± 29) | 75–94 (61 ± 27) | 3–7 (4 ± 0) | 14–30 (7 ± 2) | 58–81 (30 ± 7) | |||||
| Mannose receptor‡ | 99–100 (272 ± 68) | 38–80 (26 ± 11) | 2–7 (4 ± 0) | 24–63 (13 ± 8) | 48–95 (34 ± 20) |
*
PE-conjugated.
‡
Unconjugated antibody.
§
Results are expressed as range of values, calculated as percentage of positive cells as compared with an isotype-matched control antibody. Values in parentheses indicate the average of geometric mean fluorescence intensities of the various samples ± SD.
‖
The β3 expression in the monocytes and day 3 macrophages does not match the αvβ3 expression due to contaminating platelets that stuck to the cells during isolation, as determined by staining with anti-CD41 (data not shown).