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. 1998 Oct 5;188(7):1239–1246. doi: 10.1084/jem.188.7.1239

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Expression of Ctla-4 mRNA in thymocytes upon in vivo anti-CD3 treatment. RNA was isolated from thymus after 24 h of treatment with 100 μg of anti-CD3 or saline and analyzed by RT-PCR for Ctla-4 RNA expression. Hypoxanthine-guanine phosphoribosyl transferase (HPRT) RNA expression is shown in the lower panel as control for cDNA loading and RT-PCR. This experiment is representative of two independent RT-PCRs.