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. 1998 Oct 5;188(7):1239–1246. doi: 10.1084/jem.188.7.1239

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Intrathymic CTLA-4 blockade inhibits anti-CD3– mediated depletion of DP thymocytes. Groups of three mice were intrathymically injected with soluble anti–CTLA-4, with only saline or with anti-TNP hamster Ig as controls before administration of anti-CD3. Thymocytes were double stained with anti-CD4–PE and anti-CD8–FITC and analyzed by flow cytometry. Dot-plots represent CD4/CD8 profiles of thymocytes from (A) mice injected with saline intraperitoneally (i.p.) and intrathymically (i.t.), (B) mice injected with 100 μg anti-CD3 intraperitoneally and saline intrathymically, (C) mice injected with 100 μg anti-CD3 intraperitoneally and hamster Ig intrathymically, (D) mice injected with 100 μg anti-CD3 intraperitoneally and anti–CTLA-4 intrathymically. Percentages of DP thymocytes are shown on the upper right quadrant of each dot-plot. (E) Thymocytes recovered from mice (three mice each group) treated with increasing amounts of anti-CD3 and injected intrathymically either with anti-CTLA-4 or only saline were double stained with anti-CD4 and anti-CD8 as above. These results are expressed as the mean value ± SD and are representative of at least three independent experiments.

Intrathymic CTLA-4 blockade inhibits anti-CD3– mediated depletion of DP thymocytes. Groups of three mice were intrathymically injected with soluble anti–CTLA-4, with only saline or with anti-TNP hamster Ig as controls before administration of anti-CD3. Thymocytes were double stained with anti-CD4–PE and anti-CD8–FITC and analyzed by flow cytometry. Dot-plots represent CD4/CD8 profiles of thymocytes from (A) mice injected with saline intraperitoneally (i.p.) and intrathymically (i.t.), (B) mice injected with 100 μg anti-CD3 intraperitoneally and saline intrathymically, (C) mice injected with 100 μg anti-CD3 intraperitoneally and hamster Ig intrathymically, (D) mice injected with 100 μg anti-CD3 intraperitoneally and anti–CTLA-4 intrathymically. Percentages of DP thymocytes are shown on the upper right quadrant of each dot-plot. (E) Thymocytes recovered from mice (three mice each group) treated with increasing amounts of anti-CD3 and injected intrathymically either with anti-CTLA-4 or only saline were double stained with anti-CD4 and anti-CD8 as above. These results are expressed as the mean value ± SD and are representative of at least three independent experiments.