Figure 3.
L-selectin dimerization enhances leukocyte rolling on endothelial cells under physiologic flow. (A) Effect of coumermycin and novobiocin treatments on the number of L-Gb cells rolling on a HUVEC monolayer in an in vitro flow chamber assay. Values represent the number of L-Gb cells interacting with HUVEC monolayers in a 0.16-mm2 field. Asterisk indicates significant differences from all other groups, P < 0.01. (B) Effect of coumermycin (0.9 μM) on rolling velocities of L-Gb cells interacting with a HUVEC monolayer. Each symbol represents the velocity of an individual cell plotted in rank order with median (50%) velocities indicated by horizontal and vertical lines. (C) Effect of anti– L-selectin mAbs on the number of wild-type or L-Gb cells rolling on HUVEC monolayers. (D) Effect of anti–L-selectin mAbs on the rolling velocities of L-Gb cells interacting with HUVEC monolayers. A and C values are mean ± SEM of results obtained in three experiments, and B and D values are representative of results obtained in three experiments.