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. 1998 Oct 5;188(7):1231–1238. doi: 10.1084/jem.188.7.1231

Figure 2.

Figure 2

Sequence analysis of (A) productive and (B) nonproductive VκJκ-intron-RS rearrangements from FACS® sorted, IgM+λ+ splenic B cells. Vκ gene family and Jκ gene usage were assigned based on homologies to expressed VκJκ genes (52) or homology searches of Genbank and the Kabat Ig database. Translated Vκ FWR3 (codons 70–88), CDR3, and Jκ5 sequences to the conserved phenylalanine (F) are shown for productive rearrangements, whereas FWR3 and CDR3 sequences are shown for nonproductive rearrangements, with the asterisk (*) adjacent to CDR3 in B denoting an out of frame VκJκ join. The nucleotide sequences of the unrearranged Jκ intronic recombining sequence 1 (IRS1) and RS element (both of which contain a consensus heptamer sequence adjacent to the Δ symbol) are shown above the sequences of the IRS1-RS joins present in each PCR clone. The RS join sequence for clone 17 was not determined. Underlined nucleotides could have been donated by either the IRS1 or the RS sequence, and N region addition (bold) and P-encoded nucleotides are shown between the joins. Repeats denote the number of times a particular sequence was observed.