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. 1998 Oct 5;188(7):1231–1238. doi: 10.1084/jem.188.7.1231

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“Repair” of intron-RS recombination-silenced VκJκ genes by restoration of Cκ exon and surrounding elements reveals that silenced κL chains can pair with their original μ chain partner. The graph shows representative results from a μκ ELISA comparing several IgMκ transfectoma antibodies (Tfc) to their IgMλ parental hybridoma antibodies (Hyb). Antibodies in supernatants were captured on plastic using adsorbed anti-μ chain antibodies and revealed with anti-κ conjugates. Bars indicate the SD determined from antibodies assayed in triplicate. The concentrations of the hybridoma antibodies were at least 10-fold higher than those of the transfectoma antibodies based on comparison to a TEPC 183 (μ, κ) standard curve.