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Schematic representation of the plasmid construct used to generate Crry transgenic mice. cDNA for the signal sequence and five short consensus repeats (SCR's) of mouse Crry was subcloned into the MT 5′/3′ vector. This vector uses the mouse MT-I promoter, the human growth hormone polyadenylation site, and the mouse MT gene locus control regions contained within 5′ and 3′ hypersensitive sites. The pBluescript portion of the plasmid was removed by SalI digestion.
