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. 1998 Oct 5;188(7):1321–1331. doi: 10.1084/jem.188.7.1321

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RT-PCR showing the tissue localization of the Crry transgene mRNA. PCR was performed for 25 cycles using a 5′ primer derived from the 5′ untranslated region of the mRNA derived from the MTH promoter construct and a 3′ primer from mouse Crry sequence. As a positive control, RT-PCR for G3PDH was performed, and a negative control included RNA which was treated identically, except that reverse transcription was not performed. This is demonstrated for liver and designated −RT.