Figure 5.
Western blot analysis showing the presence of intact rsCrry in the urine of Crry transgenic animals. Urine from Crry transgenic animals was electrophoresed in lanes 2 and 4, while purified rsCrry-Ig was in lanes 1 and 3 as a positive control. Western blotting was performed with anti-Crry mAb 10A2 (lanes 1 and 2), and with anti-mouse CR1 mAb 8C12 as a negative control (lanes 3 and 4). No urinary Crry is detectable in nontransgenic mice (data not shown).