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. 1998 Nov 2;188(9):1563–1573. doi: 10.1084/jem.188.9.1563

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Effect of the steroid hormone receptor antagonist RU486 on ETR_A mRNA expression. (A) HOC were treated for 24 h with vehicle (lane 1), 10 nM dexamethasone (lane 2), 100 nM RU486 (lane 3), and dexamethasone/RU486 coincubation (lane 4). (B) HOC were incubated for a total experimental period of 24 h (changing medium after 6 h) with vehicle (lane 1) and 10 nM dexamethasone (lane 2). The other experimental groups (lane 3–5) were incubated for 6 h vehicle/18 h 100 nM RU486 (lane 3), 6 h 10 nM dexamethasone/ 18 h vehicle (lane 4), and 6 h 10 nM dexamethasone/18 h 100 nM RU486 (lane 5). Total RNA was extracted from control and treated cells. Northern blot analyses were performed with ³²P-labeled ETR_A and 28S cDNA probes. Arrows, left, 28S rRNA position. Data are representative of three experiments. Lower panels show corrected ETR_A mRNA signals in relative densitometry units expressed as percent of control.

Effect of the steroid hormone receptor antagonist RU486 on ETR_A mRNA expression. (A) HOC were treated for 24 h with vehicle (lane 1), 10 nM dexamethasone (lane 2), 100 nM RU486 (lane 3), and dexamethasone/RU486 coincubation (lane 4). (B) HOC were incubated for a total experimental period of 24 h (changing medium after 6 h) with vehicle (lane 1) and 10 nM dexamethasone (lane 2). The other experimental groups (lane 3–5) were incubated for 6 h vehicle/18 h 100 nM RU486 (lane 3), 6 h 10 nM dexamethasone/ 18 h vehicle (lane 4), and 6 h 10 nM dexamethasone/18 h 100 nM RU486 (lane 5). Total RNA was extracted from control and treated cells. Northern blot analyses were performed with ³²P-labeled ETR_A and 28S cDNA probes. Arrows, left, 28S rRNA position. Data are representative of three experiments. Lower panels show corrected ETR_A mRNA signals in relative densitometry units expressed as percent of control.