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. 1998 Nov 2;188(9):1563–1573. doi: 10.1084/jem.188.9.1563

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Effect of 10 nM dexamethasone on ETR_A mRNA decay in transcriptionally blocked HOC by actinomycin D. HOC were treated for 24 h with 10 nM dexamethasone before the addition of actinomycin D. Total RNA was extracted from control and treated cultures after 0, 2, 4, 12, and 24 h 1 μg/ ml actinomycin D treatment. RNA was analyzed by Northern blot and hybridized with ³²P-labeled ETR_A cDNA probes. Data are representative of three experiments. Ethidium bromide staining of rRNA was used to check equal RNA loading. Arrows, right, 28S and 18S rRNA positions (A). The signals were analyzed by densitometry (B), and 0-, 2-, and 4-h ETR_A mRNA levels were plotted in logarithmic scale with linear regression as a percentage of the 0-h value against time (C).

Effect of 10 nM dexamethasone on ETR_A mRNA decay in transcriptionally blocked HOC by actinomycin D. HOC were treated for 24 h with 10 nM dexamethasone before the addition of actinomycin D. Total RNA was extracted from control and treated cultures after 0, 2, 4, 12, and 24 h 1 μg/ ml actinomycin D treatment. RNA was analyzed by Northern blot and hybridized with ³²P-labeled ETR_A cDNA probes. Data are representative of three experiments. Ethidium bromide staining of rRNA was used to check equal RNA loading. Arrows, right, 28S and 18S rRNA positions (A). The signals were analyzed by densitometry (B), and 0-, 2-, and 4-h ETR_A mRNA levels were plotted in logarithmic scale with linear regression as a percentage of the 0-h value against time (C).