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. 1998 Nov 2;188(9):1633–1640. doi: 10.1084/jem.188.9.1633

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Stimulation of splenocytes from AND Tg mice with immobilized and soluble dimeric IE^K-MCC. (a) Splenocytes from AND × B10.BR Tg mice were incubated for 72 h at 37°C with various concentrations of immobilized monomeric IE^K-MCC, dimeric IE^K-MCC, or anti-CD3. Cultures were pulsed with ³H-TdR for 12 h at 37°C and uptake was measured by scintillation counting. Results are expressed as the mean of triplicate cultures (background was subtracted). (b) Strong response of splenocytes from AND Tg mice to soluble IE^K-MCC dimer. Splenocytes from AND × B10.BR Tg mice were incubated for 48 h at 37°C with various concentrations of soluble monomeric IE^K-MCC, dimeric IE^K-MCC, or anti-CD3. IL-2 present in culture supernatants was measured using ELISA. Results are expressed as the mean of triplicate cultures. One of three experiments with similar results is shown.

Stimulation of splenocytes from AND Tg mice with immobilized and soluble dimeric IE^K-MCC. (a) Splenocytes from AND × B10.BR Tg mice were incubated for 72 h at 37°C with various concentrations of immobilized monomeric IE^K-MCC, dimeric IE^K-MCC, or anti-CD3. Cultures were pulsed with ³H-TdR for 12 h at 37°C and uptake was measured by scintillation counting. Results are expressed as the mean of triplicate cultures (background was subtracted). (b) Strong response of splenocytes from AND Tg mice to soluble IE^K-MCC dimer. Splenocytes from AND × B10.BR Tg mice were incubated for 48 h at 37°C with various concentrations of soluble monomeric IE^K-MCC, dimeric IE^K-MCC, or anti-CD3. IL-2 present in culture supernatants was measured using ELISA. Results are expressed as the mean of triplicate cultures. One of three experiments with similar results is shown.