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. 1998 Nov 2;188(9):1633–1640. doi: 10.1084/jem.188.9.1633

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TCR downregulation induced by the immobilized dimer is not influenced by CD4 molecule expression. The CD4-positive and CD4-negative clones of 5KC73.8 T cell hybridoma were incubated with various concentrations of the immobilized dimer for 5 h. The percentage of TCR that remained on the cell surface after stimulation was determined as described in the legend to Fig. 4. This experiment is representative of at least three experiments with similar results.