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. 1998 Nov 2;188(9):1587–1592. doi: 10.1084/jem.188.9.1587

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Postchallenge plasma PAF and histamine levels in +/+ (filled circles) and W/W^v (open circles) mice. After challenge, blood was taken from the heart, which was cut open at the time indicated, and was mixed with a 0.1 vol of 3.8% ice-chilled citrate solution, then centrifuged immediately. Plasma PAF and histamine levels were determined as described in Materials and Methods. The results were expressed as mean ± SEM from one of two representative experiments (n = 2–4 animals for each time point), and statistical significance was determined by the Mann-Whitney U test (*P < 0.05; ^‡ P < 0.01 compared with +/+ mice).