Table 2.
Expression of Transcripts Characteristic of Mast Cells Before and After Stimulation by FcεRI
| Tag sequence* | Resting cells‡ | Activated cells§ | Gene | |||
|---|---|---|---|---|---|---|
| Mediators | ||||||
| AAACAATGTG | 49 | 28 | Proteoglycan peptide core protein | |||
| AAGTCCTGCA | 25 | 11 | Carboxypeptidase A | |||
| TGGGATCTGG | 7 | 0 | Rat mast cell protease-5 | |||
| TCCGAAGACT | 2 | 2 | Rat mast cell protease-10 | |||
| Mediator synthetases | ||||||
| CTTCAGAGGG | 11 | 19 | Histidine decarboxylase | |||
| CTACTTCTTC | 5 | 2 | 5-Lipoxygenase | |||
| CCCACATAGT | 3 | 4 | S-glutathione-S-transferase | |||
| GGCAGAGGAA | 1 | 2 | Prostaglandin D2 synthetase | |||
| CGAAATAAAT | 1 | 1 | c-Phospholipase-A2 | |||
| TGTGTCTCCA | 1 | 1 | Aromatic l-amino acid decarboxylase | |||
| Receptors | ||||||
| TTGGTTTGGG | 17 | 10 | FcεRI β | |||
| CAATAAATAA | 11 | 7 | FcεRI α | |||
| ATGCTCTGGA | 9 | 5 | FcγRIII α | |||
| CTAAGGAGAT | 6 | 3 | FcγRIIb2 α | |||
| CTAGGCTCTA | 4 | 2 | FcεRI γ |
*
In this and the succeeding tables, the tag sequences represent the 10-bp SAGE tags 3′ of the NlaIII site.
‡
In this and subsequent tables the copy numbers are from 20,177 tags from the resting cells.
§
In this and subsequent tables the copy numbers are from 20,582 tags from the activated cells.