Figure 2.

Cross-stimulation of viral peptide–primed LN cells with the self-antigen (and vice versa) can select and activate potently encephalitogenic cells. Mice were immunized with an emulsion in CFA containing either no additional antigen, 400 μg of MBP, 200 μg of pHPV-7 or MBP(87–99), or 5 μg of NP(260–83) (not shown). 10 d later, LN cells were collected and stimulated with either (A) 50 μg/ml of MBP or (B) 10 μg/ml of the specified antigens. 4 d after stimulation, 10⁸ cells were injected intraperitoneally into each naive recipient.