Table 2.

Passive Transfer of EAE with Cells that had been Primed with Either the Papillomavirus Peptide (pHPV-7), MBP(87–99), or MBP and Activated with the Specified Dose of the Priming Antigen

Immunization		In vitro stimulation		Dose		Incidence of EAE		No. of stimulations		Cells (/107) transferred		Maximal EAE score*		Day of onset*
				μg/ml
High antigen dose experiments
MBP(87–99)		MBP(87–99)		10		3/4		1		6,6,6,6		3,3,2,0		<9,<9,<9,−
pHPV-7		pHPV-7		10		0/4		1		6,6,6,6		0,0,0,0		−
MBP		MBP		25		2/2		1		6,6		2,3		8,8
PHPV-7		pHPV-7		10		0/5		1		6,6,6,6,6		0,0,0,0,0		−
MBP(87–99)		MBP(87–99)		10		4/4		1		10,10,10,10		3,3,3,3		7,7,8,8
pHPV-7		pHPV-7		50		1/6		1		10,10,10		1,0,0		9,−,−,
										10,10,10		0,0,0		−,−,−
MBP(87–99)		MBP(87–99)		10		2/3		3		2		4,4,0		7,7,0
pHPV-7		pHPV-7		10		0/3		3		2		0,0,0		−
Low antigen dose experiments
pHPV-7		pHPV-7		0.05		5/6		2		3 (1),‡ 0.6§		2,3		7,6
								2		3,3 (4)§		2,1.5		6,7
								5		3,3		2,0		8,−
pHPV-7		pHPV-7		0.05		4/5		2		3,3,0.8		2.5,2.5,0		7,7,−
								2		3 (10),§ 1§		3,2		6,7
pHPV-7		pHPV-7		0.05		4/4		2		3 (12),3,3,3		2.5,2.5,2,2		7,7,7,7
pHPV-7		pHPV-7		0.05		2/3		2		3 (16),3,3		2,1,0		8,8,−
pHPV-7		pHPV-7		0.05		4/4		2		4,4,4,4		5,3,3,2		6,6,6,8
MBP(87–99)		MBP(87–99)		0.05		4/4		2		3,3,3,3		3,2.5,2.5,2.5		5,5,6,6
MBP(87–99)		MBP(87–99)		10		4/4		2		3,3,3,3		5,5,5,4		5,5,5,5

^*Data for individual mice are separated by commas.  

^‡Numbers in parenthesis relate to histopathological samples (see Fig. 5).  

^§Enriched for T cells at the LN cell stage as described in Materials and Methods.