Figure 3.

Clonal heterogeneity in early proliferative response of single-sorted CD34⁺CD38⁻ fetal liver cells cultured in growth factor–supplemented SFM. Single CD34⁺CD38⁻ fetal liver cells were sorted into the wells of round-bottomed microtiter plates containing serum-free medium supplemented with Steel factor, Flt-3 ligand, IL-3, IL-6, and G-CSF at 37°C. At the indicated time intervals, the number of cells in each well was scored.