Table 1.
Putative ORFs in the 12.6-kb DNA Fragment
| ORF | Endpoints | Size of product | Ribsosome binding site consensus sequence and initiation codon (bold) | |||
|---|---|---|---|---|---|---|
| nt | aa | |||||
| IS30 | 1 < 402* | 383 | TGCGGAGGTTTTTTGAATG | |||
| IS911 | 362 < 727 | 122 | CAACACAGGTGCTCCAATG | |||
| orf1 | 823 > 1242 | 164 | GCCTGAAGGAGATGAAATG | |||
| hbp | 1368 > 5498 | 1377 | TCAGGAGTAATTAAAAATG | |||
| orf2 | 5610 > 6056 | 143 | ACATCTCTGGAGATTTATG | |||
| orf3 | 6486 < 7742 | 442 | GGATAAACTCCGCCGGATG | |||
| orf4 | 7943 < 8287 | 115 | TACAGGAACTGAAGCGATG | |||
| orf5 | 8926 < 9723 | 266 | GCAGGAAAAAACACGTATG | |||
| orf6 | 9860 < 10738 | 293 | CAAACAGCTCCCGTTTGTG | |||
| cvi | 10929 > 11162 | 78 | AATGGGATAAAAAGTAATG | |||
| cvaC | 11143 >11451 | 103 | ATAAAAAGGAGATCATATG | |||
| cvaB | 11627 < 12606‡ | 698 |
*
ORF has been cut at the HindIII site. Total size of the original IS30 ORF is 1,151 nucleotides.
‡
ORF has been cut at the EcoRI site. Total size of the original cvaB ORF is 2,096 nucleotides.