Figure 1.

Production of PGs by homogenates of uninfected RBCs, infected RBCs, and parasite cells. Parasite cells and infected RBCs were isolated from trophozoite- and schizont-rich FCR3 cultures as described in Materials and Methods. Uninfected RBCs were from the same RBC source used for the parasite cultures. Each cell homogenate was incubated at 37°C for 30 min in the presence or absence of 1 mM AA. The amounts of PG produced by the cell homogenates were measured by EIA and standardized to 1 ml of packed cell volume. White, light gray, and dark gray bars represent PGD₂, PGE₂, and PGF_2α, respectively.