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. 2007 Oct 8;9(5):R102. doi: 10.1186/ar2305

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Copyright © 2007 Blaney Davidson et al; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Proteoglycan content after Ad-BMP-2 injection. Knee joints of mice injected with Ad-BMP-2 or a control virus were isolated at days 3, 7, or 21 and processed for histology. Sections were stained with safranin O and fast green, after which safranin O staining intensity was measured in the articular cartilage with a computerized imaging system as a measurement of proteoglycan content of the cartilage. At least three sections per knee joint were measured. Measurements were averaged per knee joint. Statistical analysis with a Student t test. *p < 0.05. BMP-2, bone morphogenetic protein-2.