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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Neuroimmunol. 2007 Oct 24;192(1-2):57–67. doi: 10.1016/j.jneuroim.2007.09.004

Figure 5. Inflammatory cytokine and FoxP3 gene in sorted cell subsets.

Figure 5

MOG peptide primed and in vitro stimulated LN cells were stained with CD4 and CD25 antibodies as described in methods. CD4high Teff cells and CD4normalCD25+ Treg cells were sorted using gating similar to that shown in Figure 3. Gene expression from sorted cells (8 × 104 cells) was analyzed by real time PCR.