Skip to main content
. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: J Neuroimmunol. 2007 Oct 24;192(1-2):57–67. doi: 10.1016/j.jneuroim.2007.09.004

Figure 6. CD4normalCD25+ cells can suppress mitogenic T cell responses.

Figure 6

CD4normalCD25+ (Treg) cells were isolated from 4-day cultures of MOG35-55-immunized B6 MOG p35-55 lymph nodes cells by sterile sorting using gates similar to that depicted in Figure 3. Naïve CD4 positive T cells (1 × 104) were cultured for 3 days in round-bottom 96-well plates with the sorted cells and 2 × 104 APCs (T-cell-depleted splenocytes) per well in triplicate in the presence of 2.0 ug/ml of anti-CD3 antibody (clone 145-2C11) in ratios of Treg/E as indicated above. Sixteen hours before harvesting, cells were pulsed with 3H-Thymidine. Cells were harvested and incorporation of radioactive thymidine was counted in a beta- counter. Results are representative of at least two experiments.