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. 2007 Nov 26;7:104. doi: 10.1186/1471-2180-7-104

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Copyright © 2007 He et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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PCR-amplification of 2 uracil-independent transformants SOD#5 and SOD#30 using different primer pairs to confirm if the transformation construct replaced the target sequence. Lane 2–4: template DNA from transformant SOD#5 (Lane 3 in Figure 6), Lane 5–7: template DNA from transformant SOD#30 (lane 12 in Figure 6). Primer pairs used as: P1 and P6 were used on lane 2 and 5, band size is 3385 bp; Primers P0 and P6 were used on lane 3 and 6, band size is 3385 + 101 = 3486 bp; Primers P0 and P5 were used on lane 4 and 7, band size is 3385 + 101 -- 1005 = 2481 bp.