Table 2.
Primers used in this study
| Primer name | Sequence* | Purpose** |
| P0 | TTGAAGTCATGGTGGAGGTG | 1 |
| P1 | ATGTACTCCGTACTCGGTTG | 1, 2 |
| P2 | TTATATTCTCCCTCGCCATActtggaccacacgagtcaag | 1, 2 |
| P3 | cttgactcgtgtggtccaagTATGGCGAGGGAGAATATAA | 1, 2 |
| P4 | gcggaggatatttcgttttcATAACAAGGCCTCGTATGTG | 1, 2 |
| P5 | CACATACGAGGCCTTGTTATgaaaacgaaatatcctccgc | 1, 2 |
| P6 | ACTACACCTTGGGGACTGTA | 1, 2 |
* Sequence in upper-case is homologous to A. flavus genome, and lower-case is homologous to pyr4 gene of N. crassa.
** Purpose 1: identifying transformants; purpose 2: constructing overlap PCR construct.