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. 2007 Nov 2;94(4):1428–1436. doi: 10.1529/biophysj.107.117473

FIGURE 1.

FIGURE 1

TEM images of capsids formed in self-assembly reactions. Samples were stained with 2% uranyl acetate. (a) VLPs formed with 700-kDa PSS. The mean capsid size for VLPs is 22 nm. (b) VLPs formed with 3.4-MDa PSS. The mean capsid size is 27 nm. (c) Empty CCMV capsids formed by dialysis of CP in buffer with high salt and low pH. The dark core in the center indicates the penetration of stain into “void” (aqueous solution) space, which is notably absent in the interiors of VLPs filled with PSS. (d) wt CCMV capsids in virus suspension buffer. Scale bars are 50 nm.